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  • Rapid and sensitive detection of fluoroquinolone

    2007-10-15 · In this study a microarray-based assay was developed and applied, which provides a rapid, sensitive and specific detection of fluoroquinolone-resistant E. coli in urine. The capture probes were designed against previously identified and described hotspots …

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  • Quantitative detection of Escherichia coli from urine

    2012-12-1 · The PCR method made it possible to detect E. coli more quickly than the urine culture, and it proved its utility for detecting and quantitating bacteria. However, the real-time PCR method is not without problems, currently this method is expensive compared with the urine culture method.

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  • Standard urine test for cystitis is questioned | The BMJ

    2017-4-28 · The sensitive qPCR test found evidence of Escherichia coli in 95.9% of samples and Staphylococcus saprophyticus in 8.6%. Overall, the qPCR test found evidence of an infection in 98.2% of women with UTI symptoms. In the women without symptoms, standard testing picked up E coli in 10.5% of samples and qPCR picked up E coli in 11.6%.

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  • E. coli - Diagnosis and treatment - Mayo Clinic

    2010-5-28 · Practical test methods. The gold standard for a urine test is to perform a bacteriological urine culture, with identification of the pathogen, with quantification and sensitivity testing. To test whether the patient has a UTI at all, orientating indirect methods are often used in practice to detect the bacteria or inflammation (dip sticks).

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  • The Diagnosis of Urinary Tract Infection

    2012-4-30 · Dec. 7, 2020 — Researchers have developed a rapid, ultrasensitive test using a paper-based electrochemical sensor that can detect the presence of …

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  • Rapid test strips detect bacterial contamination in ...

    2010-7-27 · Urinalysis is an initial basic test of the main features of the blood. It is often part of a routine examination and is frequently performed when people are admitted to hospital and before surgery. It can also be used to check abnormal results from a rapid urine test. Complete urinalysis is done in a laboratory. It usually involves three steps:

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  • Understanding urine tests - InformedHealth.org -

    2019-11-19 · In summary, a sensitive and practical fluorometric assay has been successfully designed for the detection of E. coli O157:H7 based on fluorescence quenching. AuNPs coated with thiol oligos have significantly induced the quenching of the fluorescence signal of GQDs, which hassled to remarkable fluorescence signal changes and can be utilized for the detection of E. coli O157:H7.

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  • A fluorescence quenching based gene assay for

    2020-9-22 · Here, we describe Single MOLecule Tethering or SMOLT, an amplification-free and purification-free molecular assay that can detect microorganisms in …

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  • Ultra-sensitive and rapid detection of nucleic acids

    2) allowing the strip to remain in the urine for too long may cause leaching of reagents from the pads 3) not blotting the strip can produce a run-over between chemicals on the adjacent pads, producing distortion of the colors 4) waiting too long or not enough time to read rxns 5) not using a good lighting source 6) testing refrigerated urine

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  • Rapid and sensitive detection of fluoroquinolone

    2007-10-15 · The DNA microarray test displayed an assay time of 3.5 h, a sensitivity of 100 CFU/ml, and the ability to detect fluoroquinolone-resistant E. coli in the presence of a 10-fold excess of fluoroquinolone-susceptible E. coli cells. As a consequence, we believe that this microarray-based determination of antibiotics resistance has a true potential ...

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  • A rapid, highly sensitive and culture-free detection of ...

    2016-8-1 · Similarly, varying volumes of human whole blood were mixed with E. coli cells, and DNA was extracted in 150 μL of EB. Subsequently 25 μL of these mixed DNA and cell samples were used for each 50 μL qPCR reaction to detect E. coli. A sample with genomic DNA from 800 CFU bacteria and 1 mL blood served as a positive control.

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  • Sensitive detection of enteropathogenic E . coli using

    2013-7-23 · We have developed a sensitive assay for enteropathogenic E. coli (EPEC) by integrating DNA extraction, specific polymerase chain reaction (PCR) and DNA detection using an electrode modified with the bundle-forming pilus (bfpA) structural gene. The PCR amplified products are captured on the electrode and hybridized with biotinylated detection probes to form a sandwich hybrid …

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  • Covalent capture and electrochemical quantification

    2021-1-2 · A sensitive electrochemical sensor has been developed to covalently capture, detect, and quantify E. coli from complex sample matrices with an LOD of 12 CFU mL −1 and a linear range of detection up to 10 7 CFU mL −1. The technology enables quantification of these pathogens within two hours on disposable electrodes, even from complex ...

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  • Standard urine test for cystitis is questioned | The BMJ

    2017-4-28 · The sensitive qPCR test found evidence of Escherichia coli in 95.9% of samples and Staphylococcus saprophyticus in 8.6%. Overall, the qPCR test found evidence of an infection in 98.2% of women with UTI symptoms. In the women without symptoms, standard testing picked up E coli in 10.5% of samples and qPCR picked up E coli in 11.6%.

    Get Price
  • E. coli - Diagnosis and treatment - Mayo Clinic

    2020-10-10 · Diagnosis. To diagnose illness caused by E. coli infection, your doctor sends a sample of your stool to a laboratory to test for the presence of E. coli bacteria. The bacteria may be cultured to confirm the diagnosis and identify specific toxins, such as those produced by E. coli O157:H7.

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  • Sensitive and Rapid Detection of Chlamydia

    2014-1-1 · Chlamydia trachomatis is the most common sexually transmitted human pathogen. Infection results in minimal to no symptoms in approximately two-thirds of women and therefore often goes undiagnosed. C. trachomatis infections are a major public health concern because of the potential severe long-term consequences, including an increased risk of ectopic pregnancy, chronic pelvic pain, and …

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  • Rapid test strips detect bacterial contamination in ...

    2012-4-30 · Rapid test strips detect bacterial contamination in swimming water. Researchers have developed a rapid testing method using a simple paper strip that can detect E. coli in recreational water ...

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  • Biochemical Tests For Escherichia coli (E. coli ...

    2021-5-4 · Urine cultures can detect some sexually transmitted diseases. However, a urine culture is not the test of choice for sexually transmitted diseases in adults. Some STDs such as chlamydia may be tested using a urine sample, but the testing method used detects chlamydia genetic material in the urine and is not a culture.

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  • Rapid and specific detection of the pap, afa, and sfa ...

    These results indicate that the PCR method, which is highly specific and easier to perform than the hybridization method, is a powerful genotypic assay for detection of adhesin-encoding operons. Thus, this assay can be recommended for clinical use to detect virulent urinary E. coli strains, as well as for epidemiological studies.

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  • The Diagnosis of Urinary Tract Infection

    2010-5-28 · Practical test methods. The gold standard for a urine test is to perform a bacteriological urine culture, with identification of the pathogen, with quantification and sensitivity testing. To test whether the patient has a UTI at all, orientating indirect methods are often used in practice to detect the bacteria or inflammation (dip sticks).

    Get Price
  • Sensitive detection of enteropathogenic E . coli using

    2013-7-23 · We have developed a sensitive assay for enteropathogenic E. coli (EPEC) by integrating DNA extraction, specific polymerase chain reaction (PCR) and DNA detection using an electrode modified with the bundle-forming pilus (bfpA) structural gene. The PCR amplified products are captured on the electrode and hybridized with biotinylated detection probes to form a sandwich hybrid …

    Get Price
  • Standard urine test for cystitis is questioned | The BMJ

    2017-4-28 · The sensitive qPCR test found evidence of Escherichia coli in 95.9% of samples and Staphylococcus saprophyticus in 8.6%. Overall, the qPCR test found evidence of an infection in 98.2% of women with UTI symptoms. In the women without symptoms, standard testing picked up E coli in 10.5% of samples and qPCR picked up E coli in 11.6%.

    Get Price
  • Reliability of dipstick assay in predicting urinary tract ...

    2 天前 · The presence of oxidizing contaminants such as hypochlorite and povidone can lead to false-positive result. False negative results are seen if the dipsticks are exposed to air, pH of urine is less than 5.1 and ascorbic acid is present in the urine. Blood test was the highest sensitive single test in our study.

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  • Establishment of a highly sensitive sandwich ELISA for

    2016-12-19 · The cultured E. coli was harvested and a recombinant fusion protein of GST peptide, Titin-N (1–200) and His-tag (designated as GST-Titin-N-His) was purified using Ni-NTA agarose (Qiagen). The ...

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  • Leukocytes in urine: A sign of urinary tract infection?

    2018-7-24 · However, it is not highly sensitive, which means that the test does not pick up all bacterial infections. E. coli bacteria are most commonly associated with nitrites in the urine.

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  • Standard test may miss urinary infection in

    2017-4-28 · The researchers found that the standard test detected bacteria in 80.9 percent of urine samples from the symptomatic women. However, the qPCR test detected E. coli in …

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  • Urine Tests for Diagnosis of Infectious Diseases and ...

    2019-10-1 · The relation between disease and urine was recognized by physicians since the earliest civilization BC. Urine is considered an ideal diagnostic specimen for its noninvasive and easy method of collection. Urinalysis encompasses a wide range of tests, which includes a variety of chemical tests, urine microscopy, bacterial cultures, and molecular tests.

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  • Urinalysis Chapter 5: Chemical Examination of Urine ...

    2) allowing the strip to remain in the urine for too long may cause leaching of reagents from the pads 3) not blotting the strip can produce a run-over between chemicals on the adjacent pads, producing distortion of the colors 4) waiting too long or not enough time to read rxns 5) not using a good lighting source 6) testing refrigerated urine

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  • Urine Tests Don't Always Confirm Urinary Infections,

    There was a highly significant association between the positive results of urine dipstick of nitrite test, leukocyte esterase test, and microscopic examination for pyuria and the positive results of urine culture (P < 0.001).Furthermore, there was a highly significant association between the negative results of urine dipstick of nitrite test, leukocyte esterase test, and microscopic ...

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  • The utility of urinary dipstick in the diagnosis of ...

    E. coli Infection. E. coli normally lives in your intestines. Most strains are usually harmless. A few strains cause diarrhea/bloody diarrhea, vomiting and stomach pains and cramps. One strain can lead to kidney failure if not properly managed. Eating contaminated food is the most common way to get an E. coli …

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  • E. coli: What is It, How Does it Cause Infection,

    2021-7-21 · Dr. Mohammed Al hallak answered. 16 years experience Internal Medicine. E.coli PNA: ciprofloxacin or Levaquin usually works well . also Ceftriaxone IV or Ceftin (cefuroxime) oral works as well. we need to test the E.coli and find out ... Read More. 3 doctors agree. 0.

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  • Antibiotics sensitive to e coli | Answers from Doctors ...

    2018-7-24 · However, it is not highly sensitive, which means that the test does not pick up all bacterial infections. E. coli bacteria are most commonly associated with nitrites in the urine.

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  • How are UTIs tested? - Chronic UTI Info

    2020-8-19 · The enzyme-linked immunosorbent assay (ELISA), is the most widely used and reliable clinical routine method for the detection of important protein markers in healthcare. Improving ELISAs is crucial for detecting biomolecules relates to health disorders and facilitating diagnosis at the early diseases stages. Several methods have been developed to improve the ELISA sensitivity through ...

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  • Urease Test- Principle, Media, Procedure and Result

    2021-7-18 · Free 2-day shipping. Buy Rapid Test Complete - Urinalysis Test Strips 100 Tests UTI Strips, Kidney, Gallbladder, pH, Glucose, and Liver Function at Walmart.com

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  • Leukocytes in urine: A sign of urinary tract infection?

    Urinalysis: Chapter 5 Chemical Examination of Urine. STUDY. PLAY. Reagent Strips. Reagent strips consist of chemical-impregnated absorbent pads attached to a plastic strip used to measure medically significant substances in/conditions of urine. List 5 Problems with Reagent Strips. Improper technique can …

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  • Symptoms | E. coli | CDC

    2021-2-2 · Symptoms of Shiga toxin-producing E. coli (STEC) infection vary for each person, but often include severe stomach cramps, diarrhea (often bloody), and vomiting.Some people may have a fever, which usually is not very high (less than 101˚F/38.5˚C). Most people get better within 5 to 7 days.

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  • Bacterial Microcompartment-Mediated Ethanolamine ...

    2019-7-23 · Urinary tract infections (UTIs) are common and in general are caused by intestinal uropathogenic Escherichia coli (UPEC) ascending via the urethra. Microcompartment-mediated catabolism of ethanolamine, a host cell breakdown product, fuels the competitive overgrowth of intestinal E. coli, both pathogenic enterohemorrhagic E. coli and commensal strains. During a UTI, urease-negative E. coli ...

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  • Leukocytes in urine: A sign of urinary tract infection?

    2018-7-24 · However, it is not highly sensitive, which means that the test does not pick up all bacterial infections. E. coli bacteria are most commonly associated with nitrites in the urine.

    Get Price
  • Urine dipsticks - Don`t Forget The Bubbles

    2021-6-8 · Nitrites are the breakdown product by gram-negative organisms such as E.coli. They are a more specific test (93-98%) than leukocytes but their sensitivity is lower (47-49%). The sensitivity is particularly poor as the urine needs to sit in the bladder for a while (at least 4 hours) for it to be positive.

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  • E. coli and food safety - Mayo Clinic

    2020-1-29 · Foods that have been linked to E. coli include beef, sprouts, spinach, lettuce, ready-to-eat salads, fruit, raw milk, and raw flour and cookie dough.. To protect yourself from E. coli infection and other foodborne illnesses, follow basic food safety guidelines:. Wash your hands, utensils and kitchen surfaces with hot, soapy water before and after preparing or eating food.

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  • Antibiotic Susceptibility Testing | Lab Tests Online

    2018-5-22 · Knowing this, a practitioner may rely on current susceptibility patterns for E. coli to choose an antibiotic that is likely to be effective in most cases. In addition, there are certain life-threatening infections, such as meningitis , that must be treated immediately, with no time to …

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  • Highly enhanced ELISA sensitivity using acetylated ...

    2020-8-19 · The enzyme-linked immunosorbent assay (ELISA), is the most widely used and reliable clinical routine method for the detection of important protein markers in healthcare. Improving ELISAs is crucial for detecting biomolecules relates to health disorders and facilitating diagnosis at the early diseases stages. Several methods have been developed to improve the ELISA sensitivity through ...

    Get Price
  • Are You Highly Sensitive? – The Highly Sensitive Person

    Scoring: If you answered more than fourteen of the questions as true of yourself, you are probably highly sensitive. But no psychological test is so accurate that an individual should base his or her life on it. We psychologists try to develop good questions, then decide on the cut off based on the average response.

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  • Urinalysis: Chapter 5 Chemical Examination of Urine ...

    Urinalysis: Chapter 5 Chemical Examination of Urine. STUDY. PLAY. Reagent Strips. Reagent strips consist of chemical-impregnated absorbent pads attached to a plastic strip used to measure medically significant substances in/conditions of urine. List 5 Problems with Reagent Strips. Improper technique can …

    Get Price
  • Rapid Test Complete - Urinalysis Test Strips 100 Tests

    2021-7-18 · Free 2-day shipping. Buy Rapid Test Complete - Urinalysis Test Strips 100 Tests UTI Strips, Kidney, Gallbladder, pH, Glucose, and Liver Function at Walmart.com

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